The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• may correspond to ca. 10 MPa, assuming 1 nN loading force and 5 nm tip radius. Though the resulting artificial depression depths on the cellular membranes are about half a micrometer already at 1 nN, AFM is useful to measure the cortical actin network underneath the membrane [18]. Regarding the

Internalization mechanisms of cell-penetrating peptides

• Ivana Ruseska and
• Andreas Zimmer

Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• expanded absorptive surface, which facilitates rapid absorption of digestive products [4], but also constitutes an effective barrier against microorganisms, pathogens and foreign substances [5]. Moreover, assembly of the F-actin network in the brush border occurs due to expression and recruitment of actin

• -binding proteins [6]. The main proteins involved are fimbrin and villin, whereby the latter one is the key component and determines organization and plasticity of the F-actin network [7][8]. In contrast, M cells show no brush border with only sparse irregular microvilli [9][10]. Interestingly, in M cells

• different number of microvilli reflects an altered organization of the F-actin network between M cells and Caco-2 cells labeling of cytoskeletal F-actin-fibers with rhodamine-phalloidin was performed. In Caco-2 cells, an intense F-actin labeling at the apex of the cells was obtained, indicating a fully

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• actin networks and talin into model cells. Keywords: actin network; cell adhesion; giant unilamellar vesicle; integrin; lipid bilayer; synthetic cell; protein reconstitution; talin; Review Introduction Since Hooke first described a biological cell in 1665 tremendous progress has been made in

• was taken further by assembling dendritic actin networks inside GUVs to study the interaction between actin network growth and deformation of membranes [67]. It was observed that actin-based protrusions formed inside the GUVs, which showed a strong resemblance to cellular filopodia (Figure 5). Liu and

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• play the main role in the mechanical properties of living cells [15][31]. The observed increase of cellular deformability, which is induced by cytochalasin D, relates to the mechanical properties of the cytoskeleton with the actin network lying beneath the cell membrane. However, it cannot be specified